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human lung bronchial epithelial cell line (hbe)  (Boster Bio)


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    Boster Bio human lung bronchial epithelial cell line (hbe)
    CSE enhanced HE4 expression in <t>HBE</t> cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial <t>epithelial;</t> HPF, human pulmonary fibroblast
    Human Lung Bronchial Epithelial Cell Line (Hbe), supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung bronchial epithelial cell line (hbe)/product/Boster Bio
    Average 90 stars, based on 1 article reviews
    human lung bronchial epithelial cell line (hbe) - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease"

    Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

    Journal: Respiratory Research

    doi: 10.1186/s12931-022-02040-7

    CSE enhanced HE4 expression in HBE cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial epithelial; HPF, human pulmonary fibroblast
    Figure Legend Snippet: CSE enhanced HE4 expression in HBE cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial epithelial; HPF, human pulmonary fibroblast

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    HE4 affected IL-6 release in HBE cells through phosphorylation of NFκB-p65. A–C The transfection of siRNA in HBE cells significantly lowered HE4 mRNA expression (n = 3), secretory level (n = 3) and intracellular protein expression (n = 4). A , B, D Infecting lentivirus expressing HE4 markedly enhanced HE4 mRNA expression (n = 3), secretory level (n = 4) and intracellular protein expression (n = 4). The western blot images were shown and quantified by ImageJ. E, G HE4 knockdown mitigated IL-6 expression while HE4 overexpression augmented IL-6 expression in HBE cells both in mRNA level (n = 3) and secretory protein level (n = 4). F, H Intervening HE4 expression had no significant impact on IL-8 expression, although reducing HE4 expression can alleviate CSE-induced IL-8 elevation at mRNA level (n = 3–4). I, J Knockdown of HE4 expression reduced phosphorylation of NFκB-p65 relative to the expression of p65 (n = 4). K, L Overexpression of HE4 increased phosphorylation of NFκB-p65 (n = 5). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences. HE4, human epididymis protein 4; si, small interfering RNA; NC, negative control; Lv-GFP, lentivirus with empty vector carrying green fluorescent protein; Lv-HE4, lentivirus expressing HE4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; IL-6, interleukin-6; IL-8, interleukin-8
    Figure Legend Snippet: HE4 affected IL-6 release in HBE cells through phosphorylation of NFκB-p65. A–C The transfection of siRNA in HBE cells significantly lowered HE4 mRNA expression (n = 3), secretory level (n = 3) and intracellular protein expression (n = 4). A , B, D Infecting lentivirus expressing HE4 markedly enhanced HE4 mRNA expression (n = 3), secretory level (n = 4) and intracellular protein expression (n = 4). The western blot images were shown and quantified by ImageJ. E, G HE4 knockdown mitigated IL-6 expression while HE4 overexpression augmented IL-6 expression in HBE cells both in mRNA level (n = 3) and secretory protein level (n = 4). F, H Intervening HE4 expression had no significant impact on IL-8 expression, although reducing HE4 expression can alleviate CSE-induced IL-8 elevation at mRNA level (n = 3–4). I, J Knockdown of HE4 expression reduced phosphorylation of NFκB-p65 relative to the expression of p65 (n = 4). K, L Overexpression of HE4 increased phosphorylation of NFκB-p65 (n = 5). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences. HE4, human epididymis protein 4; si, small interfering RNA; NC, negative control; Lv-GFP, lentivirus with empty vector carrying green fluorescent protein; Lv-HE4, lentivirus expressing HE4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; IL-6, interleukin-6; IL-8, interleukin-8

    Techniques Used: Phospho-proteomics, Transfection, Expressing, Western Blot, Knockdown, Over Expression, Small Interfering RNA, Negative Control, Plasmid Preparation

    Elevated HE4 expression in CSE-exposed HBE cells was mediated by oxidative stress. A Pretreatment of NAC overtly alleviated CSE-induced HE4 increase (n = 3). B Different concentrations of H 2 O 2 promoted HE4 expression (n = 3). C Pretreating NAC significantly mitigated H 2 O 2 -induced HE4 up-regulation (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05 and **P < 0.01 represent significant differences. HE4, human epididymis protein 4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; DMSO, dimethyl sulfoxide; NAC, N-acetylcysteine
    Figure Legend Snippet: Elevated HE4 expression in CSE-exposed HBE cells was mediated by oxidative stress. A Pretreatment of NAC overtly alleviated CSE-induced HE4 increase (n = 3). B Different concentrations of H 2 O 2 promoted HE4 expression (n = 3). C Pretreating NAC significantly mitigated H 2 O 2 -induced HE4 up-regulation (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05 and **P < 0.01 represent significant differences. HE4, human epididymis protein 4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; DMSO, dimethyl sulfoxide; NAC, N-acetylcysteine

    Techniques Used: Expressing



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    CSE enhanced HE4 expression in <t>HBE</t> cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial <t>epithelial;</t> HPF, human pulmonary fibroblast
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    CSE enhanced HE4 expression in HBE cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial epithelial; HPF, human pulmonary fibroblast

    Journal: Respiratory Research

    Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

    doi: 10.1186/s12931-022-02040-7

    Figure Lengend Snippet: CSE enhanced HE4 expression in HBE cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial epithelial; HPF, human pulmonary fibroblast

    Article Snippet: Human lung bronchial epithelial cell line (HBE) was obtained from the Boster Biotech Co., Ltd (Wuhan, China).

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    HE4 affected IL-6 release in HBE cells through phosphorylation of NFκB-p65. A–C The transfection of siRNA in HBE cells significantly lowered HE4 mRNA expression (n = 3), secretory level (n = 3) and intracellular protein expression (n = 4). A , B, D Infecting lentivirus expressing HE4 markedly enhanced HE4 mRNA expression (n = 3), secretory level (n = 4) and intracellular protein expression (n = 4). The western blot images were shown and quantified by ImageJ. E, G HE4 knockdown mitigated IL-6 expression while HE4 overexpression augmented IL-6 expression in HBE cells both in mRNA level (n = 3) and secretory protein level (n = 4). F, H Intervening HE4 expression had no significant impact on IL-8 expression, although reducing HE4 expression can alleviate CSE-induced IL-8 elevation at mRNA level (n = 3–4). I, J Knockdown of HE4 expression reduced phosphorylation of NFκB-p65 relative to the expression of p65 (n = 4). K, L Overexpression of HE4 increased phosphorylation of NFκB-p65 (n = 5). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences. HE4, human epididymis protein 4; si, small interfering RNA; NC, negative control; Lv-GFP, lentivirus with empty vector carrying green fluorescent protein; Lv-HE4, lentivirus expressing HE4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; IL-6, interleukin-6; IL-8, interleukin-8

    Journal: Respiratory Research

    Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

    doi: 10.1186/s12931-022-02040-7

    Figure Lengend Snippet: HE4 affected IL-6 release in HBE cells through phosphorylation of NFκB-p65. A–C The transfection of siRNA in HBE cells significantly lowered HE4 mRNA expression (n = 3), secretory level (n = 3) and intracellular protein expression (n = 4). A , B, D Infecting lentivirus expressing HE4 markedly enhanced HE4 mRNA expression (n = 3), secretory level (n = 4) and intracellular protein expression (n = 4). The western blot images were shown and quantified by ImageJ. E, G HE4 knockdown mitigated IL-6 expression while HE4 overexpression augmented IL-6 expression in HBE cells both in mRNA level (n = 3) and secretory protein level (n = 4). F, H Intervening HE4 expression had no significant impact on IL-8 expression, although reducing HE4 expression can alleviate CSE-induced IL-8 elevation at mRNA level (n = 3–4). I, J Knockdown of HE4 expression reduced phosphorylation of NFκB-p65 relative to the expression of p65 (n = 4). K, L Overexpression of HE4 increased phosphorylation of NFκB-p65 (n = 5). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences. HE4, human epididymis protein 4; si, small interfering RNA; NC, negative control; Lv-GFP, lentivirus with empty vector carrying green fluorescent protein; Lv-HE4, lentivirus expressing HE4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; IL-6, interleukin-6; IL-8, interleukin-8

    Article Snippet: Human lung bronchial epithelial cell line (HBE) was obtained from the Boster Biotech Co., Ltd (Wuhan, China).

    Techniques: Phospho-proteomics, Transfection, Expressing, Western Blot, Knockdown, Over Expression, Small Interfering RNA, Negative Control, Plasmid Preparation

    Elevated HE4 expression in CSE-exposed HBE cells was mediated by oxidative stress. A Pretreatment of NAC overtly alleviated CSE-induced HE4 increase (n = 3). B Different concentrations of H 2 O 2 promoted HE4 expression (n = 3). C Pretreating NAC significantly mitigated H 2 O 2 -induced HE4 up-regulation (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05 and **P < 0.01 represent significant differences. HE4, human epididymis protein 4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; DMSO, dimethyl sulfoxide; NAC, N-acetylcysteine

    Journal: Respiratory Research

    Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

    doi: 10.1186/s12931-022-02040-7

    Figure Lengend Snippet: Elevated HE4 expression in CSE-exposed HBE cells was mediated by oxidative stress. A Pretreatment of NAC overtly alleviated CSE-induced HE4 increase (n = 3). B Different concentrations of H 2 O 2 promoted HE4 expression (n = 3). C Pretreating NAC significantly mitigated H 2 O 2 -induced HE4 up-regulation (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05 and **P < 0.01 represent significant differences. HE4, human epididymis protein 4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; DMSO, dimethyl sulfoxide; NAC, N-acetylcysteine

    Article Snippet: Human lung bronchial epithelial cell line (HBE) was obtained from the Boster Biotech Co., Ltd (Wuhan, China).

    Techniques: Expressing